Journal: Chinese Medicine

Article Title: Single-cell RNA sequencing reveals the therapeutic mechanism of Calvatia lilacina in promoting wound healing of anal fistula

doi: 10.1186/s13020-025-01293-w

Figure Lengend Snippet: The impact of CLS on inflammation, angiogenesis, and collagen at different time points. A – C ELISA detection of TNF-α, VEGF, and Collagen I expression levels in granulation tissue; D Immunohistochemical staining of CD14 and CD11b; E , F Immunohistochemical quantitative analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns: not significant ( P > 0.05) vs.the control group; n = 10

Article Snippet: TNF-α, COL1A1, VEGF Elisa Kit (EK182, EK183, EK1C01, Multi Sciences, China); WASF3 Antibody (67620-1-lg, Proteintech, China); α-SMA (19245s, CST, USA); CD14 Antibody (ab183322, abcam, Britain); Cluster of Differentiation 11b (CD11b) Antibody (ab133357, abcam, Britain); IL-6 flow Antibody (562050, BD Pharmingen, USA); JAK2 (WL02188, Wanleibio, China); p-JAK2 (WL02997, Wanleibio, China); STAT3 (WL03207, Wanleibio, China); p-STAT3 (WL06214, Wanleibio, China);

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemical staining, Staining, Control

Journal: Chinese Medicine

Article Title: Single-cell RNA sequencing reveals the therapeutic mechanism of Calvatia lilacina in promoting wound healing of anal fistula

doi: 10.1186/s13020-025-01293-w

Figure Lengend Snippet: Flow cytometry and SEM detection of adhesion and effect of CLS on macrophages. A Flow cytometry was used to determine the ratio of IL-6 positive cells in macrophage after extraction. B Quantitative analysis the rate of IL-6 positive macrophage. C , D Elisa detection of IL-6 and CXCL-8 in cell supernatant. E , F Intensity of intercellular communication between control group and treatment group. G Analysis of IL-6 + macrophage and fibroblast receptor-ligand binding. H Analysis of cell population interaction intensity of IL-6, CXCL-8 signaling pathway. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant ( P > 0.05) vs.the control group; n = 3

Article Snippet: TNF-α, COL1A1, VEGF Elisa Kit (EK182, EK183, EK1C01, Multi Sciences, China); WASF3 Antibody (67620-1-lg, Proteintech, China); α-SMA (19245s, CST, USA); CD14 Antibody (ab183322, abcam, Britain); Cluster of Differentiation 11b (CD11b) Antibody (ab133357, abcam, Britain); IL-6 flow Antibody (562050, BD Pharmingen, USA); JAK2 (WL02188, Wanleibio, China); p-JAK2 (WL02997, Wanleibio, China); STAT3 (WL03207, Wanleibio, China); p-STAT3 (WL06214, Wanleibio, China);

Techniques: Flow Cytometry, Extraction, Enzyme-linked Immunosorbent Assay, Control, Ligand Binding Assay